Positions

ASSOCIATE IN RESEARCH

Animal Surgery - Regenerative Medicine for Cardiac and Skeletal Muscle Disease

We are looking for an individual with strong experience in animal surgery and handling. The main research interests of the Bursac group are in cell and gene therapy applications for cardiac and skeletal muscle injury and disease. For cardiac regeneration research, experiments will involve induction of myocardial infarction and transverse aortic constriction in mice and rats, followed by implantation of a bioengineered cardiac tissue patch or injection of therapeutic cells or viruses, as well as assistance with histological and physiological studies to assess therapeutic effects, including echocardiographic measurements. For skeletal muscle regeneration studies, experiments will involve generation of a large volumetric muscle loss injuries in mice followed by implantation of engineered muscle tissues or injection of therapeutic viruses at the site of muscle injury as well as assistance with physiological measurements and histological studies to assess therapeutic effects. This position will also involve writing and maintenance of related animal and safety protocols.

Qualifications include BS, MS, PhD, MD, DVM, or equivalent degrees in physiology, veterinary medicine, medicine, or other relevant areas of biomedical sciences. The ideal candidate will possess strong surgical training in small animals, be highly self-motivated and able to do independent troubleshooting. Experience with heart and muscle injury models and related physiological studies will be given priority. The Bursac research group and Duke University is a highly diverse and stimulating environment that provides excellent opportunities for scientific growth in the pursuit of a variety of careers. Salary will be commensurate with experience.

 

Research Technician -  Cardiac Tissue Engineering

Associate in Research position is immediately available in the Cardiac Electrophysiology and Tissue Engineering laboratory of Biomedical Engineering Department at Duke University under Professor Nenad Bursac (http://bursaclab.pratt.duke.edu/). Responsibilities include isolation and culture of neonatal rat cardiomyocytes, maintenance of cell lines, molecular cloning and mutagenesis, production of adenoviruses and lentiviruses, cell transfections and transductions, qRT-PCR, immunofluorescence staining, and immunoblotting. Responsibilities also include handling of mice and rats. Should be able to work alone and in teams, independently review related scientific literature to troubleshoot encountered problems and improve on used methodologies. 

Required qualifications: B.S. degree in biology or related field with experience in cell culture, molecular biology, and biochemical techniques. Applications will be reviewed as they are received until candidate is selected. The training environment of the Bursac group is highly interdisciplinary and provides ample opportunities for scientific growth and pursuit of both academic and industry careers. Duke University is an equal opportunity employer. Interested candidates should send their resume and names of three reference writers to Dr. Nenad Bursac (nbursac@duke.edu).

Postdoctoral Fellows

Postdoctoral Fellow in Cell and Gene Therapy for Cardiac Arrhythmias

Postdoctoral openings in gene- and cell-based therapy for cardiac arrhythmias and myocardial infarction are immediately available in the Bursac lab. We are looking for highly motivated candidates with expertise in cell and molecular biology, single cell electrophysiology, optical mapping of action potential propagation in Langendorff-perfused hearts, and small or large animal studies. The project involves development of novel in situ and exogenous cell- and gene-based therapies for cardiac arrhythmias and will include work in ion channel engineering, genetic manipulation of excitable and unexcitable cells, and application of tissue and genetic engineering techniques to improve electrical conduction and prevent or terminate arrhythmias in injured or chronically diseased hearts. Post-myocardial infarction induced VT and chronic AF will be the two main applications of this work.

Qualifications include PhD and 0-3 years of postdoctoral experience in single cell and tissue/organ cardiac electrophysiology, molecular biology, genetics, and other relevant areas of biomedical sciences. The ideal candidate will be highly self-motivated and possess strong training in electrophysiology, molecular biology, animal studies, biochemical and histological assays. Candidates with experience in optical mapping, generation of transgenic animals, and genome editing (CRISPR/Cas9) techniques will be given a preference. Postdoctoral fellows are expected to publish research findings in peer-reviewed journals, participate in conferences for the intellectual exchange of research ideas, engage in existing and new collaborations within and outside Duke University, and assist with training of undergraduate and graduate students, as needed. The environment of the Bursac group is highly interdisciplinary and provides ample opportunities for scientific growth and pursuit of both academic and industry careers. Interested candidates should send their resume, statement of research goals, and at least three names for recommendation letters to Dr. Nenad Bursac (nbursac@duke.edu)

Postdoctoral Fellow in Genome Editing for Striated Muscle Regeneration

Immediate postdoctoral openings in genome editing for regeneration of striated (cardiac or skeletal) muscle are available in the Bursac lab. We are looking for highly motivated candidates with experience in genome engineering technology, including CRISPR/Cas9 editing, and next generation sequencing. The main therapeutic application will involve endogenous or stem cell-based regeneration of infarcted heart and injured or diseased skeletal muscle. Postdoctoral fellows are expected to generate new human pluripotent stem cell lines by CRISPR/Cas9 editing, perform gRNA library screening to identify candidate effector genes, and differentiate hiPSCs into the cells of cardiovascular, skeletal muscle, or immune system lineage. Tissue engineering of human cardiac and skeletal muscle from hiPS cells will be further