Quantifying electrical interactions between cardiomyocytes and other cells in micropatterned cell pairs

TitleQuantifying electrical interactions between cardiomyocytes and other cells in micropatterned cell pairs
Publication TypeJournal Article
Year of Publication2014
AuthorsNguyen, H, Badie, N, McSpadden, L, Pedrotty, D, and Bursac, N
JournalMethods in molecular biology (Clifton, N.J.)
Volume1181
Start Page249
Pagination249 - 262
Date Published01/2014
Abstract

Micropatterning is a powerful technique to control cell shape and position on a culture substrate. In this chapter, we describe the method to reproducibly create large numbers of micropatterned heterotypic cell pairs with defined size, shape, and length of cell-cell contact. These cell pairs can be utilized in patch clamp recordings to quantify electrical interactions between cardiomyocytes and non-cardiomyocytes. © 2014 Springer Science+Business Media New York.

DOI10.1007/978-1-4939-1047-2-21
Short TitleMethods in molecular biology (Clifton, N.J.)