Title | Quantifying electrical interactions between cardiomyocytes and other cells in micropatterned cell pairs |
Publication Type | Journal Article |
Year of Publication | 2014 |
Authors | H Nguyen, N Badie, L McSpadden, D Pedrotty, and N Bursac |
Journal | Methods in Molecular Biology (Clifton, N.J.) |
Volume | 1181 |
Start Page | 249 |
Pagination | 249 - 262 |
Date Published | 01/2014 |
Abstract | Micropatterning is a powerful technique to control cell shape and position on a culture substrate. In this chapter, we describe the method to reproducibly create large numbers of micropatterned heterotypic cell pairs with defined size, shape, and length of cell-cell contact. These cell pairs can be utilized in patch clamp recordings to quantify electrical interactions between cardiomyocytes and non-cardiomyocytes. © 2014 Springer Science+Business Media New York. |
DOI | 10.1007/978-1-4939-1047-2-21 |
Short Title | Methods in Molecular Biology (Clifton, N.J.) |