Quantifying electrical interactions between cardiomyocytes and other cells in micropatterned cell pairs

Abstract

Micropatterning is a powerful technique to control cell shape and position on a culture substrate. In this chapter, we describe the method to reproducibly create large numbers of micropatterned heterotypic cell pairs with defined size, shape, and length of cell-cell contact. These cell pairs can be utilized in patch clamp recordings to quantify electrical interactions between cardiomyocytes and non-cardiomyocytes. © 2014 Springer Science+Business Media New York.

DOI
10.1007/978-1-4939-1047-2-21
Year