Functional reentry in cultured monolayers of neonatal rat cardiac cells.

TitleFunctional reentry in cultured monolayers of neonatal rat cardiac cells.
Publication TypeJournal Article
Year of Publication2003
AuthorsS Iravanian, Y Nabutovsky, C-R Kong, S Saha, N Bursac, and L Tung
JournalAmerican journal of physiology. Heart and circulatory physiology
Volume285
Issue1
Start PageH449
PaginationH449 - H456
Date Published07/2003
Abstract

Previous studies of reentrant arrhythmias in the heart have been performed in computer models and tissue experiments. We hypothesized that confluent monolayers of cardiac cells can provide a simple, controlled, and reproducible experimental model of reentry. Neonatal rat ventricular cells were cultured on 22-mm-diameter coverslips and stained with the voltage-sensitive dye RH-237. Recordings of transmembrane potentials were obtained from 61 sites with the use of a contact fluorescence imaging system. An electrical field stimulus, followed by a point stimulus, induced 39 episodes of sustained reentry and 21 episodes of nonsustained reentry. Sustained reentry consisted of single-loop (n = 18 monolayers) or figure-of-eight (n = 4) patterns. The cycle length, action potential duration at 80% repolarization, and conduction velocity were (in means +/- SE) 358 +/- 33 ms, 118 +/- 12 ms, and 12.9 +/- 1.0 cm/s for single loop and 311 +/- 78 ms, 137 +/- 18 ms, and 7.8 +/- 1.3 cm/s for figure-of-eight, respectively. Electrical termination by 6- to 13-V/cm field pulses or 15- to 20-V point stimuli was successful in 60% of the attempts. In summary, highly stable reentry can be induced, sustained for extensive periods of time, and electrically terminated in monolayers of cultured neonatal rat cardiac myocytes.

DOI10.1152/ajpheart.00896.2002
Short TitleAmerican journal of physiology. Heart and circulatory physiology